Yakachena Artemisia capillaris mafuta ekenduru uye sipo kugadzira wholesale diffuser yakakosha mafuta matsva ekupisa tsanga diffuser.

Yakachena Artemisia capillaris mafuta ekenduru nesipo kugadzira wholesale diffuser yakakosha mafuta matsva ekupisa tsanga diffuser Featured Image

tsananguro pfupi:

Rodent model design

Mhuka idzi dzakakamurwa zvisina tsarukano kuita mapoka mashanu emakonzo gumi neshanu rimwe nerimwe. Boka rekutonga uye mhando dzeboka mbeva dzakagarwa nesesame mafutakwemazuva matanhatu. Positive control group mbeva dzakaiswa gavaged nemapiritsi e-bifendate (BT, 10 mg/kg) kwemazuva matanhatu. Mapoka ekuedza akabatwa ne 100 mg / kg uye 50 mg / kg AEO yakanyungudutswa muSesame mafuta kwemazuva matanhatu. Pazuva rechitanhatu, boka rekutonga rakabatwa nemafuta eesame, uye mamwe mapoka ose akabatwa nedoro rimwe re0.2% CCl4 mumafuta esame (10 ml / kg) ne.intraperitoneal jekiseni. Mbeva dzakabva dzatsanya pasina mvura, uye sampuli dzeropa dzakatorwa kubva mumidziyo yeretrobulbar; ropa rakaunganidzwa raive centrifuged pa 3000 ×gkwe 10 min kuti iparadzanise serum.Cervical dislocationyakaitwa pakarepo mushure mekubviswa kweropa, uye samples dzechiropa dzakabviswa nokukurumidza. Chimwe chikamu chechiropa sampuro chakabva chachengetwa pa -20 ° C kusvika pakuongororwa, uye chimwe chikamu chakabviswa uye chakagadziriswa mu10%formalinmhinduro; matishu akasara akachengetwa pa -80 °C kuti aongorore histopathological (Wang nevamwe, 2008,Hsu et al., 2009,Nie et al., 2015).

Kuyera kweiyo biochemical parameters muserum

Kukuvara kwechiropa kwakaongororwa nekufungidziraenzymatic mabasayeSerum ALT uye AST uchishandisa iyo inoenderana yekutengesa kits zvinoenderana nemirairo yemakiti (Nanjing, Jiangsu Province, China). Iyo enzymatic zviitiko zvakaratidzwa sezvikamu parita (U / l).

Kuyera kweMDA, SOD, GSH uye GSH-Pxmuropa homogenates

Matishu echiropa akaiswa homogenized neinotonhora physiological saline pachiyero che1: 9 (w / v, chiropa: saline). Iwo mahomogenates aive centrifuged (2500 ×gkwe10 min) kuunganidza mashura ezvisarudzo zvinotevera. Kukuvara kwechiropa kwakaongororwa maererano nezviyero zvehepatic zveMDA neGSH mazinga pamwe neSOD neGSH-P.xmabasa. Zvese izvi zvakatemerwa kutevedzera mirairo pakiti (Nanjing, Jiangsu Province, China). Mhedzisiro yeMDA neGSH yakaratidzwa se nmol per mg protein (nmol/mg prot), uye mabasa eSOD neGSH-P.xzvakaratidzwa seU per mg protein (U/mg prot).

Histopathological analysis

Zvikamu zvechiropa chichangobva kuwanikwa zvakagadziriswa mu10% yakavharwaparaformaldehydephosphate solution. Sample yakazoiswa muparafini, yakachekwa kuita 3-5 μm zvikamu, zvakasvibiswa ne.hematoxylinuyeeosin(H&E) zvinoenderana neyakajairwa maitiro, uye pakupedzisira yakaongororwa namicroscope yakareruka(Tian et al., 2012).

Statistical analysis

Mhedzisiro yacho yakaratidzwa seyanoreva ± standard deviation (SD). Mhedzisiro yacho yakaongororwa pachishandiswa nhamba yehurongwa SPSS Statistics, vhezheni 19.0. Iyo data yakaiswa pasi pekuongororwa kwekusiyana (ANOVA,p<0.05) ichiteverwa nebvunzo yaDunnett uye bvunzo yaDunnett T3 kuti vaone kusiyana kwakakosha pakati pezvakakosha zvemapoka akasiyana ekuedza. Musiyano wakakosha wakatariswa pamwero wep<0.05.

Migumisiro uye nhaurirano

Zvikamu zveAEO

Pamusoro peGC / MS kuongororwa, iyo AEO yakawanikwa iine zvikamu makumi maviri neshanu zvakaburitswa kubva ku10 kusvika ku35 min, uye zvikamu makumi maviri neshanu zvinoverengera 84% yemafuta akakosha zvakaonekwa.Tafura 1) Mafuta anopisa aivemomonoterpenoids(80.9%), sesquiterpenoids (9.5%), saturated unbranched hydrocarbons (4.86%) uye zvakasiyana-siyana acetylene (4.86%). Kuenzaniswa nezvimwe zvidzidzo (Guo et al., 2004), takawana akawanda monoterpenoids (80.90%) muAEO. Mhedzisiro yacho yakaratidza kuti iyo yakanyanya kuwanda yeAEO ndeye β-citronellol (16.23%). Zvimwe zvikamu zvikuru zveAEO zvinosanganisira 1,8-cineole (13.9%),camphor(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),thymol(3.22%) uyemyrcene(2.02%). Musiyano mukuumbwa kwemakemikari unogona kunge uchienderana nemamiriro ekunze ayo chirimwa chakafumurwa, senge mineral water, chiedza chezuva, nhanho yekukura uye.chikafu.

FOB Mutengo:US $0.5 - 9,999 / Piece

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Supply Ability:10000 Piece / Zvimedu pamwedzi

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Chirwere chechiropa, chirwere chinowanzoitika chinokonzerwa neviral hepatitis, kudhakwa, makemikari ane chepfu chiropa, tsika dzisina kunaka dzekudya uye kusvibiswa kwezvakatipoteredza, chinhu chinonetsa pasi rose (Papay et al., 2009) Zvisinei, kurapwa kwechirwere ichi kunowanzova kwakaoma kutungamirira uye kune simba rakaganhurirwa. Traditional Chinesemishonga yemakwenzi, iyo iri pasi pemirairo yakawanda inoshandiswa kurapa zvirwere zvechiropa, ichiri kushandiswa zvakanyanya nemaChinese (Zhao et al., 2014)Artemisia capillarisThunb.,Asteraceae, maererano neBencao Gangmu, zvinyorwa zvakakurumbira zveChinese Traditional Medicine, zvakashandiswa zvakanyanya semushonga wekubvisa kupisa, kukurudzira.diuresisuye bvisa jaundice uye yakashandiswawo sechinhu chinonhuwira muzvinwiwa, miriwo, uye makeke nekuda kwekunhuhwirira kwayo.A. capillarisyave ichionekwa semhando yemishonga yechivanhu yechiChinese uye chikafu nenhamba iri kukura yevanhu. Nokudaro, kwave kune kuedza kukuru kugadzira mishonga yemiti inobatsira, yakadai seA. capillaris, kuitira kurapwa kwechirwere chechiropa.

Mumakore achangopfuura, mishonga yemakwenzi yakawedzera kutariswa uye kufarirwa kwekurapa kwechirwere chechiropa nekuda kwekuchengetedza uye kushanda kwayo (Ding et al., 2012)A. capillarisyakaratidza kuve ine yakanaka hepatoprotective chiitiko chinoenderana neazvino nzira dzemishonga (Han et al., 2006) Icho chinhu chakakosha chemishonga muChina uye inozivikanwa anti-kuzvimba (Cha et al., 2009a),choleretic(Yoon naKim, 2011uye anti-bundu (Feng et al., 2013)mushonga wemishonga.

Phytochemicalzvidzidzo zvakaratidza huwandu hwemafuta anokosha anokosha,coumarins,uyeflavonol glycosidespamwe chete neboka revanhu vasingazivikanwiaglyconeskubvaA. capillaris(Komiya et al., 1976,Yamahara et al., 1989) Mafuta anokosha eA. capillaris(AEO) ndeimwe yeakanyanya pharmacological anoshanda komisheni uye inopa anti-kuzvimba (Cha et al., 2009a) uye anti-apoptotic properties (Cha et al., 2009b) Nekudaro, seAEO ndeimwe yemakomisheni makuru eA. capillaris, iyo inogona kuita hepatoprotective mabasa ezvikamu zvikuru kubvaA. capillarisinofanira kuongororwa.

Muchidzidzo ichi, kudzivirira maitiro eAEO pacarbon tetrachloride(CCl4) -inducedhepatotoxicityyakaongororwa nenzira dzebiochemical, dzakadai sehepaticyakaderedzwa glutathione(GSH),malondialdehyde(MDA) mazinga,superoxide dismutase(SOD), uyeglutathione peroxidase(GSH-Px) chiitiko, pamwe nemabasa easpartate aminotransferase(AST) uyealanine aminotransferase(ALT) mu serum. Kuwanda kweCCl4-induced chiropa kukuvara kwakaongororwawo kuburikidza ne histopathological observations, pamwe chete ne phytochemical analysis neGC-MS kuti ione zvikamu zveAEO.