Osteoarthritis (OA) ndechimwe chenguva refu chisingaperi chisingaperi chechirwere chemapfupa chejoini chinobata vanhu vakwegura vanopfuura 65 [
1]. Kazhinji, varwere veOA vanoonekwa kuti vane cartilage yakakuvadzwa, inoputika synovium, uye eroded chondrocytes, izvo zvinokonzera marwadzo uye kushungurudzika mumuviri [
2]. Marwadzo eArthritic anonyanya kukonzerwa nekuderera kwecartilage mumajoini nekuzvimba, uye kana cartilage yakuvadzwa zvakanyanya mapfupa anogona kudhumhana zvichikonzera kurwadziwa kusingatsungirike uye kuoma kwemuviri [
3]. Kubatanidzwa kwevamiriri vezvipembenene vane zviratidzo zvakadai sekurwadziwa, kuzvimba, uye kuoma kwekubatana kwakanyatsonyorwa. Mune varwere veOA, cytokines inoputika, iyo inokonzera kuputika kwecartilage uye subchondral bone inowanikwa mu synovial fluid.
4]. Zvichemo zviviri zvikuru izvo varwere veOA vanowanzova nazvo kurwadziwa uye kuzvimba kwe synovial. Naizvozvo zvibodzwa zvekutanga zveazvino OA marapiro ndeekudzikisa marwadzo nekuzvimba. [
5]. Kunyange zvazvo mishonga yeOA iripo, kusanganisira isiri-steroidal uye steroidal zvinodhaka, zvakaratidza kushanda mukuderedza marwadzo uye kuzvimba, kushandiswa kwenguva refu kwemishonga iyi kune hutano hwakanyanya hwehutano hwakadai semwoyo, gastro-intestinal, uye renal dysfunctions [
6]. Saka, mushonga unoshanda une mashoma mhedzisiro unofanirwa kugadzirwa pakurapa osteoarthritis.
Zvigadzirwa zvehutano zvechisikigo zviri kuwedzera kufarirwa kuve zvakachengeteka uye kuwanikwa zviri nyore [
7]. Mishonga yechinyakare yekuKorea yakaratidza kushanda kwayo kurwisa zvirwere zvakawanda zvinopisa, kusanganisira arthritis [
8]. Aucklandia lappa DC. inozivikanwa nemishonga yayo, yakadai sekusimudzira kutenderera kwe qi yekubvisa marwadzo nekunyaradza dumbu, uye yave ichishandiswa sechinyakare seanalgesic yechisikigo [
9]. Mishumo yapfuura inoratidza kuti A. lappa ine anti-inflammatory [
10,
11], analgesic [
12], anticancer [
13], uye gastroprotective [
14] migumisiro. Zvinhu zvakasiyana-siyana zvehupenyu zveA lappa zvinokonzerwa nemishonga yayo mikuru inoshanda: costunolide, dehydrocostus lactone, dihydrocostunolide, costuslactone, α-costol, saussurea lactone uye costuslactone [
15]. Zvidzidzo zvepakutanga zvinoti costunolide yakaratidza anti-inflammatory properties mu lipopolysaccharide (LPS), iyo yakakonzera macrophages kuburikidza nekugadzirisa kweNF-kB uye kupisa kunotyisa kweprotein nzira [
16,
17]. Zvakadaro, hapana ongororo yakaongorora zvinogona kuitika zveA. lappa pakurapwa kweOA. Tsvagiridzo yazvino yakaongorora marapirwo eA. lappa kurwisa OA vachishandisa (monosodium-iodoacetate) MIA neacetic acid-induced rodent modhi.
Monosodium-iodoacetate (MIA) inonyanya kushandiswa kugadzira maitiro mazhinji ekurwadziwa uye pathophysiological maficha eOA mumhuka.
18,
19,
20]. Kana ichiiswa mumajoini emabvi, MIA inoparadza chondrocyte metabolism uye inokonzera kuzvimba uye zviratidzo zvinoputika, zvakadai sekartilage uye subchondral bone erosion, cardinal zviratidzo zveOA [
18]. Writhing mhinduro inokonzerwa neacetic acid inotaridzirwa zvakanyanya sekuenzanisa kwekurwadziwa kwepamuviri mumhuka umo kurwadziwa kwekuputika kunogona kuyerwa zvakanyanya [
19]. Iyo mbeva macrophage cell line, RAW264.7, inonyanya kushandiswa kudzidza mhinduro dzemaserura pakuzvimba. Paunenge uchishandiswa neLPS, RAW264 macrophages inoshandisa nzira dzekuvhiringidza uye inobudisa vatsigiri vakawanda vanopisa, seTNF-α, COX-2, IL-1β, iNOS, uye IL-6 [
20]. Ichi chidzidzo chakaongorora maitiro e-anti-nociceptive uye anopesana nekuputika kweA. lappa inopesana neOA muMIA mhuka yemuenzaniso, acetic acid-induced animal model, uye LPS-activated RAW264.7 masero.
2. Zvishandiso uye Nzira
2.1. Plant Material
Mudzi wakaomeswa weA. lappa DC. yakashandiswa mukuyedza yakatengwa kubva kuEpulip Pharmaceutical Co., Ltd., (Seoul, Korea). Yakaonekwa naProf. Donghun Lee, Dhipatimendi reHerbal pharmacology, Col. weKorea Medicine, Gachon University, uye nhamba yevhoti yemuenzaniso yakaiswa se18060301.
2.2. HPLC Analysis yeA. lappa Extract
A. lappa yakabudiswa uchishandisa reflux apparatus (mvura yakasvibiswa, 3 h pa100 ° C). Mushonga wakabudiswa wakanatswa uye wakaderedzwa uchishandisa low-pressure evaporator. A. lappa extract yakava negoho re44.69% mushure mekuomesa nechando pasi pe −80 °C. Chromatographic analysis yeA. lappa yakaitwa neHPLC yakabatana uchishandisa 1260 InfinityⅡ HPLC-system (Agilent, Pal Alto, CA, USA). Pakuparadzaniswa kwechromatic, EclipseXDB C18 column (4.6 × 250 mm, 5 µm, Agilent) yakashandiswa pa35 °C. Zvose zve 100 mg yemuenzaniso yakaderedzwa mu 10 mL ye 50% methanol uye sonicated ye 10 min. Samples dzakasefa nesirinji sefa (Waters Corp., Milford, MA, USA) ye0.45 μm. Kuumbwa kwechikamu chekufambisa kwaiva 0.1% phosphoric acid (A) uye acetonitrile (B) uye koramu yakabudiswa sezvinotevera: 0-60 min, 0%; 60–65 min, 100%; 65–67 min, 100%; 67-72 min, 0% solvent B ine kuyerera kwe 1.0 mL / min. Iyo tsvina yakaonekwa pa210 nm uchishandisa jekiseni vhoriyamu ye10 μL. Ongororo yacho yakaitwa mumatatu.
2.3. Kugara Kwemhuka uye Kutarisira
Murume Sprague-Dawley (SD) makonzo ane mavhiki e5 uye mbeva dzechirume dzeICR dzine masvondo matanhatu dzakatengwa kubva kuSamtako Bio Korea (Gyeonggi-do, Korea). Mhuka dzaichengetwa mukamuri uchishandisa tembiricha nguva dzose (22 ± 2 ° C) uye humidity (55 ± 10%) uye chiedza / rima kutenderera kwe12/12 h. Mhuka idzi dzaizivikanwa nemamiriro acho kweinopfuura vhiki isati yatanga kuedza. Mhuka dzaive nead libitum yekudya uye mvura. Mitemo yezvino yehutsika yekuchengetwa kwemhuka uye kubata paGachon University (GIACUC-R2019003) yakatevedzwa zvakatsiga mune ese mhuka yekuedza maitiro. Chidzidzo chacho chakagadzirirwa muongorori-akapofumadzwa uye kuyedza kwakafanana. Isu takatevera nzira ye euthanasia maererano nemirairo ye Animal Experimental Ethics Committee.
2.4. MIA Jekiseni uye Kurapa
Makonzo akapatsanurwa zvisina tsarukano kuita mapoka mana, anoti sham, control, indomethacin, uye A. lappa. Kuve anesthetized ne 2% isofluorane O2 musanganiswa, makonzo akaiswa jekiseni achishandisa 50 μL yeMIA (40 mg / m; Sigma-Aldrich, St. Louis, MO, USA) intra-articularly mumabvi akabatanidzwa kuti atungamirire kuedza OA. Mishonga yakaitwa senge pazasi: kutonga uye mapoka esham aichengetwa chete neAIN-93G yakakosha kudya. Chete, boka reindomethacin rakapiwa indomethacin (3 mg/kg) yakabatanidzwa muAIN-93G kudya uye A. lappa 300 mg/kg boka rakapiwa kuAIN-93G kudya kwakawedzerwa neA. lappa (300 mg/kg). Mishonga yakaenderera mberi kwemazuva makumi maviri nemana kubva pazuva reOA induction pamwero we15-17 g pa190-210 g uremu hwemuviri pazuva.
2.5. Kuyera Kutakura Kuyera
Mushure meOA induction, kuyerwa kwehuremu hwehuremu hwemashure emakonzo akaitwa ne incapacitance-MeterTester600 (IITC Life Science, Woodland Hills, CA, USA) sezvakarongwa. Huremu kugoverwa pamakumbo eshure kwakaverengerwa: huremu hwekuremerwa simba (%)
